Potential Immunoinflammatory Role of Staphylococcal Enterotoxin A in Atopic Dermatitis: Immunohistopathological Analysis and in vitro Assay
Annals of Dermatology
;
: 173-180, 2013.
Artigo
em Inglês
| WPRIM
| ID: wpr-148973
ABSTRACT
BACKGROUND:
The underlying mechanism of atopic dermatitis (AD) exacerbated by Staphylococcus aureus has not been established. However, we demonstrated recently that the majority of S. aureus strains colonized in the skin of Korean AD patients carried genes encoding staphylococcal enterotoxin A (SEA) and/or toxic shock syndrome toxin-1 (TSST-1).OBJECTIVE:
To clarify the role of staphylococcal superantigen, SEA in AD.METHODS:
With the lesional skin of 9 AD patients and normal looking skin of one healthy adult, we examined first the expression of SEA, staphylococcal enterotoxin B (SEB), and TSST-1 using immunohistochemical analysis. In addition, we investigated the effects of SEA on the expression of inflammation-related adhesion molecules and cytokines in human HaCaT keratinocytes and Human Umbilical Vein Endothelial Cells (HUVECs) by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis and enzyme-linked immunosorbent assay.RESULTS:
Staphylococcal protein A (SPA) and SEA were detected with increased immunoreactivity in AD patients. However, TSST-1 showed mild-to-moderate immunoreactivity in AD patients, whereas SEB was minimally detected. In the double immunofluorescence investigation, SEA and SPA were well co-localized. SEA induced upregulation of adhesion molecules and elicited inflammatory responses in HaCaT keratinocytes and HUVECs.CONCLUSION:
This study demonstrates the importance of SEA as an immunoinflammatory triggering factor of AD in Koreans.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Choque Séptico
/
Pele
/
Proteína Estafilocócica A
/
Staphylococcus aureus
/
Toxinas Bacterianas
/
Queratinócitos
/
Regulação para Cima
/
Citocinas
/
Imunofluorescência
/
Superantígenos
Limite:
Adulto
/
Humanos
Idioma:
Inglês
Revista:
Annals of Dermatology
Ano de publicação:
2013
Tipo de documento:
Artigo
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