15-Lipoxygenase-1 Mediates Mucociliary Differentiation in Normal Human Nasal Epithelial Cells
Journal of Rhinology
;
: 92-96, 2005.
Artigo
em Coreano
| WPRIM
| ID: wpr-149124
ABSTRACT
BACKGROUND AND OBJECTIVES:
15-lipoxygenase-1 (15-LO-1) is involved in the differentiation of human tracheobronchial epithelial cells. Here we investigated the relation between 15-LO-1 expression and the differentiation of normal human nasal epithelial (NHNE) cells. MATERIALS ANDMETHODS:
NHNE cells, RT-PCR, Western blot analysis, and scanning electron microscopy (SEM) were used.RESULTS:
In retinoic acid (RA)-sufficient culture media, 15-LO-1 expression in NHNE cells increased time-dependently, but its expression was undetectable in RA-deficient culture media. Moreover, in RA-deficient culture media, IL-4 time-dependently induced 15-LO-1 expression at a concentration of 1 ng/mL. In addition, MUC8 gene expression, a marker of mucociliary differentiation, was up-regulated by 15-LO-1, which was itself induced by IL-4. In SEM, the ciliated epithelium was observed with the treatment of IL-4.CONCLUSION:
Our findings suggest that 15-LO-1 may be related to the differentiation of human nasal epithelium, and that it may mediate the mucociliary differentiation of NHNE cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Tretinoína
/
Microscopia Eletrônica de Varredura
/
Expressão Gênica
/
Diferenciação Celular
/
Western Blotting
/
Interleucina-4
/
Cílios
/
Meios de Cultura
/
Células Epiteliais
/
Epitélio
Limite:
Humanos
Idioma:
Coreano
Revista:
Journal of Rhinology
Ano de publicação:
2005
Tipo de documento:
Artigo
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