Fracture Healing Effects of Locally-Administered Adipose Tissue-Derived Cells
Yonsei Medical Journal
;
: 1106-1113, 2015.
Artigo
em Inglês
| WPRIM
| ID: wpr-150472
ABSTRACT
PURPOSE:
Although the applications of adipose tissue-derived cells (ADCs) in regenerative medicine have been investigated, the role of ADCs in fracture healing remains unclear. In this study, we examined the fracture-healing effects and survival of transplanted ADCs using micro-computed tomography (CT) and bioluminescence imaging (BLI). MATERIALS ANDMETHODS:
Luciferase-expressing ADCs were suspended in solubilized basement membrane preparation (SBMP) and xenografted on defects in the right femur of nude mice (n=5). SBMP alone was grafted on a defect in the contralateral femur. Serial in vivo micro-CT and BLI were performed for 20 days. Ex vivo BLI images of both femurs were obtained. Differences in the Hounsfield unit (HU), HUratio, and luciferase activities were compared using Wilcoxon signed-rank tests and non-parametric longitudinal analyses (p<0.05).RESULTS:
In vivo BLI revealed a signal drop on day 2, reconstitution on day 5, and continuous decrement thereafter. Ex vivo BLI revealed residual activity in the ADC-implanted and adjacent areas. No activity was detected in the contralateral femur. The overall increment rate of normalized HUs was higher for ADC-treated femurs than for SBMP-treated femurs. Cell migration to distant injury sites was not detected.CONCLUSION:
Enhanced bone density in the implant area suggests that ADCs have fracture-healing effects.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Tomografia Computadorizada por Raios X
/
Movimento Celular
/
Células Cultivadas
/
Tecido Adiposo
/
Consolidação da Fratura
/
Microtomografia por Raio-X
/
Fêmur
/
Camundongos Nus
Limite:
Animais
Idioma:
Inglês
Revista:
Yonsei Medical Journal
Ano de publicação:
2015
Tipo de documento:
Artigo
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