The Action Mechanism of Growth Hormone on Regeneration of Nitiric Oxide Synthase(NOS)-containing Nerves after Cavernous Neurotomy in the Rat / 대한비뇨기과학회지
Korean Journal of Urology
; : 1043-1050, 1999.
Article
em Ko
| WPRIM
| ID: wpr-150601
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE: As growth hormone was reported to improve cavernosal nerve regeneration, we studied the action mechanism of growth hormone(GH) on the regeneration of nitric oxide synthase(NOS)-containing penile nerves after unilateral cavernous nerve neurotomy in rats. MATERIALS AND METHODS: Male rats were divided into three groups: sham operation(n=10); unilateral neurotomy of a 5mm segment of the cavernous nerve(n=10) and unilateral neurotomy with GH injection(n=10). Electrostimulation of the intact cavernous nerve was performed at 1 and 3 months after operation. Nicotinamide adenine dinucleotide phosphate(NADPH) diaphorase staining was used to identify nNOS in penile nerve fibers of the mid-shaft segment. The gene expression for nNOS, insulin like growth factor(IGF)-I and nerve growth factor(NGF) were investigated in corporal tissue by reverse transcriptase-polymerase chain reaction(RT-PCR) using specific oligonucleotide primers. RESULTS: Electrostimulation in the GH-treated group revealed a greater maximal intracavernosal pressure and a shorter latency period than in those given neurotomy alone at 3 months after operation. One month after unilateral neurotomy, both neurotomy alone and the GH-treated groups showed a significant decrease in NOS-containing nerve fibers in the dorsal and intracavernosal nerves on the side of neurotomy, however mRNA expression of nNOS and IGF-I showed a significant increase in GH-treated group. At 3 months, the number of NOS-containing nerve fibers in the neurotomy alone group did not increase while the GH-treated group showed a significant increase. CONCLUSIONS: These results show that GH significantly enhances the regeneration of NOS-containing fibers in the dorsal and intracavernosal nerves after unilateral cavernous nerve injury via IGF-I.
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WPRIM
Assunto principal:
Regeneração
/
RNA Mensageiro
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Fator de Crescimento Insulin-Like I
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Hormônio do Crescimento
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Expressão Gênica
/
Primers do DNA
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Insulina
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Período de Latência Psicossexual
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NAD
/
Fibras Nervosas
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Humans
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Male
Idioma:
Ko
Revista:
Korean Journal of Urology
Ano de publicação:
1999
Tipo de documento:
Article