Effect of Nitric Oxide on the Proliferation of Cultured Porcine Trabecular Meshwork Cells
Korean Journal of Ophthalmology
;
: 1-6, 2003.
Artigo
em Inglês
| WPRIM
| ID: wpr-152372
ABSTRACT
To investigate the effect of nitric oxide (NO) on the proliferation of trabecular meshwork (TM) cells, primarily cultured porcine TM cells were exposed to NO donor (SNAP, -nitroso-N-acetyl-D, L-penicillamine) with and without its inhibitor (L-NAME, N (w) -Nitro-L-arginine methyl ester). The proliferation of TM cells was quantified by a rapid colorimetric assay. Acridine orange/Hoechest 33342 staining and flow cytometry with annexin-PI were done. As a result, NO inhibited the proliferation of TM cells significantly in a dose-dependent manner and this inhibitory effect was abolished by L-NAME. Fluorescent microscopy and flow cytometric analysis revealed that NO induced apoptotic cell death. The current results suggest that NO inhibit the proliferation of TM cells and apoptosis may be involved in some degree.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Suínos
/
Malha Trabecular
/
Benzimidazóis
/
Laranja de Acridina
/
Divisão Celular
/
Sobrevivência Celular
/
Células Cultivadas
/
Doadores de Óxido Nítrico
/
S-Nitroso-N-Acetilpenicilamina
/
Citometria de Fluxo
Limite:
Animais
Idioma:
Inglês
Revista:
Korean Journal of Ophthalmology
Ano de publicação:
2003
Tipo de documento:
Artigo
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