Production of Recombinant Buckwheat Allergen / 소아알레르기및호흡기학회지
Pediatric Allergy and Respiratory Disease
;
: 215-220, 1998.
Artigo
em Coreano
| WPRIM
| ID: wpr-152460
ABSTRACT
PURPOSE:
Buckwheat is one of the most common food allegen in Korea and frequently elicit severe allergic reactions. However, up to now, only few reports on buckwheat allergens have been reproted. The purpose of this study was to isolate mRNA for the production of recombinant buckwheat allergens.METHODS:
After the isolation of mRNA from ripening buckwheat seeds, in vitro translation was performed. The proteim patters of in vitro traslate products were identified using SDS-PAGE.RESULTS:
A260/A260 ratio total RNA was 1.9 and mRNA was 2.0. In vitro translate products showed towered molecular buckwheat proteins such as 1, 3, and 14kD, while other high molecular weight protein of buckwheat seed were not shown in SDS-PAGE.CONCLUSION:
mRNA of buckwheat was purified only from ripening seeds. The composition of mRNA was different according to the ripening periods. It is believed that this finding can give a clue to the basic research of buckwheat allergen.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
RNA
/
RNA Mensageiro
/
Alérgenos
/
Fagopyrum
/
Eletroforese em Gel de Poliacrilamida
/
Hipersensibilidade
/
Coreia (Geográfico)
/
Peso Molecular
Tipo de estudo:
Estudo prognóstico
País/Região como assunto:
Ásia
Idioma:
Coreano
Revista:
Pediatric Allergy and Respiratory Disease
Ano de publicação:
1998
Tipo de documento:
Artigo
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