Lysophosphatidic acid induces cell migration through the selective activation of Akt1
Experimental & Molecular Medicine
;
: 445-452, 2008.
Artigo
em Inglês
| WPRIM
| ID: wpr-153292
ABSTRACT
Akt plays pivotal roles in many physiological responses including growth, proliferation, survival, metabolism, and migration. In the current studies, we have evaluated the isoform-specific role of akt in lysophosphatidic acid (LPA)-induced cell migration. Ascites from ovarian cancer patients (AOCP) induced mouse embryo fibroblast (MEF) cell migration in a dose-dependent manner. On the other hand, ascites from liver cirrhosis patients (ALCP) did not induce MEF cell migration. AOCP-induced MEF cell migration was completely blocked by pre-treatment of cells with LPA receptor antagonist, Ki16425. Both LPA- and AOCP-induced MEF cell migration was completely attenuated by PI3K inhibitor, LY294002. Furthermore, cells lacking Akt1 displayed defect in LPA-induced cell migration. Re-expression of Akt1 in DKO (Akt1(-/-)Akt2(-/-)) cells restored LPA-induced cell migration, whereas re-expression of Akt2 in DKO cells could not restore the LPA-induced cell migration. Finally, Akt1 was selectively phosphorylated by LPA and AOCP stimulation. These results suggest that LPA is a major factor responsible for AOCP-induced cell migration and signaling specificity of Akt1 may dictate LPA-induced cell migration.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Neoplasias Ovarianas
/
Ascite
/
Especificidade por Substrato
/
Lisofosfolipídeos
/
Movimento Celular
/
Células Cultivadas
/
Embrião de Mamíferos
/
Ativação Enzimática
/
Proteínas Proto-Oncogênicas c-akt
/
Fosfatidilinositol 3-Quinase
Limite:
Animais
/
Feminino
/
Humanos
/
Gravidez
Idioma:
Inglês
Revista:
Experimental & Molecular Medicine
Ano de publicação:
2008
Tipo de documento:
Artigo
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