IGF-1 Counteracts TGF-beta-Mediated Enhancement of Fibronectin for in Vitro Human Lens Epithelial Cells
Yonsei Medical Journal
;
: 949-954, 2007.
Artigo
em Inglês
| WPRIM
| ID: wpr-154658
ABSTRACT
PURPOSE:
To determine whether insulin-like growth factor (IGF-1) affects transforming growth factor (TGF-beta)- mediated fibronectin accumulation in human lens epithelial cell line (HLE B-3) cells. MATERIALS ANDMETHODS:
HLE B-3 cells were incubated for 24 hours with TGF-beta (10ng/ mL), IGF-1 (10ng/mL), or both. Expression of the fibronectin gene was determined using a real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Fibronectin levels were examined using western blot analysis and immunofluorescence staining.RESULTS:
Expression of the fibronectin gene was not different between the TGF-beta/IGF-1 treated group and the TGF-beta treated group (p= 0.116). However, western blot analysis demonstrated decreased fibronectin levels in human lens epithelial cells treated with TGF-beta and IGF-1 compared to those treated with TGF-beta only (p < 0.01). Immunofluorescence staining disclosed inhibition of TGF-beta-induced fibronectin in the presence of IGF-1.CONCLUSION:
This study suggests that IGF-1 counteracts TGF-beta-mediated fibronectin accumulation in human lens epithelial cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fator de Crescimento Insulin-Like I
/
Linhagem Celular
/
Regulação da Expressão Gênica
/
Fator de Crescimento Transformador beta
/
Imunofluorescência
/
Fibronectinas
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Células Epiteliais
/
Cristalino
Limite:
Humanos
Idioma:
Inglês
Revista:
Yonsei Medical Journal
Ano de publicação:
2007
Tipo de documento:
Artigo
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