Synergistic Effect of Parthenolide in Combination with 5-Fluorouracil in SW480 Cells
Intestinal Research
;
: 357-364, 2012.
Artigo
em Coreano
| WPRIM
| ID: wpr-154834
ABSTRACT
BACKGROUND/AIMS:
Parthenolide (PT) is responsible for the bioactivities of Feverfew. Besides its potent anti-inflammatory effect, this compound has recently been reported to induce apoptosis in cancer cells. Unfortunately, many of the therapies that use 5-fluorouracil (5-FU) alone or in combination with other agents are likely to become ineffective due to drug resistance. In the present study, we investigate the antitumor effect of PT combined with 5-FU on colorectal cancer cells.METHODS:
SW480 cell was employed as a representative of human colorectal carcinoma (CRC) cells. We performed MTT, annexin-V assay, and Hoechst 33258 staining to measure the synergistic effect. Western blotting was used to demonstrate apoptotic pathway.RESULTS:
Our result demonstrated that PT inhibited the viability of colorectal cancer cells and had synergistic anti-proliferation in combination with 5-FU. After combined treatment of 5-FU and PT, enhanced apoptotic cell death is observed using annexin-V FITC assay and it was revealed by the condensed chromatin and fragmented DNA. Compared with 5-FU or PT alone, the apoptosis of colorectal cancer cells treated with PT and 5-FU enhanced the activation of caspase-8, caspase-3.CONCLUSIONS:
Combined treatment with PT may offer an efficacious strategy to overcome 5-FU resistance in certain CRC cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sesquiterpenos
/
DNA
/
Resistência a Medicamentos
/
Cromatina
/
Neoplasias Colorretais
/
Western Blotting
/
Fluoresceína-5-Isotiocianato
/
Morte Celular
/
Apoptose
/
Tanacetum parthenium
Limite:
Humanos
Idioma:
Coreano
Revista:
Intestinal Research
Ano de publicação:
2012
Tipo de documento:
Artigo
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