Tryptase and Protease-Activated Receptor 2 Expression Levels in Irritable Bowel Syndrome
Gut and Liver
;
: 382-390, 2016.
Artigo
em Inglês
| WPRIM
| ID: wpr-155145
ABSTRACT
BACKGROUND/AIMS:
Previous studies have revealed that mast cells (MCs) may activate the protease-activated receptors and release of neuropeptides involved in the pathogenesis of irritable bowel syndrome (IBS). The levels of protease-activated receptor 2 (PAR-2) and tryptase can contribute to understanding the pathogenesis of IBS.METHODS:
Colonoscopic biopsies were performed of 38 subjects (20 with IBS-diarrhea [IBS-D], eight with IBS-constipation [IBS-C], and 10 healthy volunteers). The mRNA and protein levels of tryptase and PAR-2 were assessed by real-time PCR and Western blot. The levels of vasoactive intestinal peptide (VIP), substance P (SP), and calcitonin gene-related peptide (CGRP) were measured by immunohistochemistry, and MCs were counted by toluidine blue staining.RESULTS:
Significant increases in the mRNA expression of tryptase (p<0.05, IBS-D, IBS-C vs control) and PAR-2 (p<0.05, IBS-D, IBS-C vs control) and in the tryptase protein level (p<0.05, IBS-D, IBS-C vs control) were detected in IBS. Elevations of MCs, CGRP, VIP and SP (p<0.05, IBS-D vs control) were observed for IBS-D only.CONCLUSIONS:
Tryptase levels may upregulate the function of PAR-2, resulting in the release of neuropeptide and they were correlated with clinical symptoms associated with IBS.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Cloreto de Tolônio
/
Biópsia
/
Neuropeptídeos
/
RNA Mensageiro
/
Peptídeo Intestinal Vasoativo
/
Imuno-Histoquímica
/
Substância P
/
Peptídeo Relacionado com Gene de Calcitonina
/
Western Blotting
/
Síndrome do Intestino Irritável
Idioma:
Inglês
Revista:
Gut and Liver
Ano de publicação:
2016
Tipo de documento:
Artigo
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