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Ethanol Extract of Peanut Sprout Exhibits a Potent Anti-Inflammatory Activity in Both an Oxazolone-Induced Contact Dermatitis Mouse Model and Compound 48/80-Treated HaCaT Cells
Annals of Dermatology ; : 142-151, 2015.
Artigo em Inglês | WPRIM | ID: wpr-156730
ABSTRACT

BACKGROUND:

We developed an ethanol extract of peanut sprouts (EPS), a peanut sprout-derived natural product, which contains a high level of trans-resveratrol (176.75 microg/ml) and was shown to have potent antioxidant activity.

OBJECTIVE:

We evaluated the potential anti-inflammatory activity of EPS by measuring its antioxidant potential in skin.

METHODS:

The anti-inflammatory activity of EPS was tested using two models of skin inflammation oxazolone (OX)-induced contact dermatitis in mice and compound 48/80-treated HaCaT cells. As biomarkers of skin inflammation, cyclooxygenase-2 (COX-2) and nerve growth factor (NGF) levels were measured.

RESULTS:

OX-induced contact dermatitis was suppressed markedly in mice that were treated with an ointment containing 5% EPS as evidenced by a decrease in the extent of scaling and thickening (p<0.05) and supported by a histological study. COX-2 (messenger RNA [mRNA] and protein) and NGF (mRNA) levels, which were upregulated in the skin of OX-treated mice, were suppressed markedly in the skin of OX+EPS-treated mice. Consistent with this, compound 48/80-induced expression of COX-2 (mRNA and protein) and NGF (mRNA) in HaCaT cells were suppressed by EPS treatment in a dose-dependent manner. As an inhibitor of NF-kappaB, IkappaB protein levels were dose-dependently upregulated by EPS. Fluorescence-activated cell sorting (FACS) analysis revealed that EPS scavenged compound 48/80-induced reactive oxygen species (ROS) in HaCaT cells.

CONCLUSION:

EPS exerts a potent anti-inflammatory activity via its anti-oxidant activity in both mouse skin and compound 48/80-treated HaCaT cells in vitro. Compound 48/80-treated HaCaT cells are a useful new in vitro model of skin inflammation.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Pele / RNA / Biomarcadores / Oxazolona / NF-kappa B / Espécies Reativas de Oxigênio / P-Metoxi-N-metilfenetilamina / Fator de Crescimento Neural / Dermatite de Contato / Etanol Tipo de estudo: Estudo prognóstico Limite: Animais Idioma: Inglês Revista: Annals of Dermatology Ano de publicação: 2015 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Pele / RNA / Biomarcadores / Oxazolona / NF-kappa B / Espécies Reativas de Oxigênio / P-Metoxi-N-metilfenetilamina / Fator de Crescimento Neural / Dermatite de Contato / Etanol Tipo de estudo: Estudo prognóstico Limite: Animais Idioma: Inglês Revista: Annals of Dermatology Ano de publicação: 2015 Tipo de documento: Artigo