Mutational Analysis of the Epidermal Growth Factor Receptor Gene in Gastrointestinal Stromal Tumors
Journal of the Korean Gastric Cancer Association
;
: 268-271, 2004.
Artigo
em Coreano
| WPRIM
| ID: wpr-157461
ABSTRACT
PUPOSE Most gastrointestinal stromal tumors (GISTs) have gain-of-function mutations of the KIT or the platelet-derived growth factor receptor alpha (PDGFRA) genes, but approximately 10% of the GISTs are wild types for both the KIT and the PDGFRA genes. The purpose of this study was to investigate the possibility that epidermal growth factor receptor (EGFR) gene mutation might be responsible for the pathogenesis of GIST. MATERIALS AND METHODS:
We analyzed the EGFR gene in 60 GISTs for the detection of somatic mutations by using the polymerase chain reaction (PCR), the single strand conformation polymorphism (SSCP), and DNA sequencing in exon 18, 19, and 21 encoding the kinase domain.RESULTS:
The SSCP analysis revealed no evidence of EGFR mutations in exon 18, 19, and 21 in GISTs.CONCLUSION:
The data indicate that the EGFR gene may not be mutated in human GIST and suggest that therapies targeting the mutated EGFR gene products might not be useful in the treatment of GISTs.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fosfotransferases
/
Reação em Cadeia da Polimerase
/
Éxons
/
Análise de Sequência de DNA
/
Receptores do Fator de Crescimento Derivado de Plaquetas
/
Polimorfismo Conformacional de Fita Simples
/
Genes erbB-1
/
Tumores do Estroma Gastrointestinal
/
Fator de Crescimento Epidérmico
/
Receptores ErbB
Limite:
Humanos
Idioma:
Coreano
Revista:
Journal of the Korean Gastric Cancer Association
Ano de publicação:
2004
Tipo de documento:
Artigo
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