Apocynin preserves glutamatergic neurons in the basolateral amygdala in mice with neonatal sevoflurane exposure / 대한마취과학회지
Korean Journal of Anesthesiology
;
: 335-340, 2017.
Artigo
em Inglês
| WPRIM
| ID: wpr-158005
ABSTRACT
BACKGROUND:
Neonatal exposure to anesthetics induces neuronal apoptosis and long-term cognitive dysfunction in rodents. We showed that the nicotinamide adenine dinucleotide phosphate-oxidase inhibitor apocynin not only reduces neurotoxicity by decreasing superoxide levels and preventing mitochondrial dysfunction but also improves long-term memory impairment in neonatal mice exposed to sevoflurane. We also found that after the contextual fear conditioning test, glutamatergic neurons expressed c-Fos (neural activation) regardless of previous exposure to sevoflurane. Moreover, there were fewer c-Fos-expressing glutamatergic neurons in the basolateral amygdala (BLA) after exposure to sevoflurane than after exposure to carrier gas. In this study, we investigated whether the administration of apocynin prior to sevoflurane exposure would preserve glutamatergic neurons in the BLA.METHODS:
Apocynin (50 mg/kg) was injected intraperitoneally into six-day-old male mice 30 min before 6 h of exposure to 3% sevoflurane or carrier gas only. The mice were allowed to mature and then were subjected to the contextual fear conditioning test. The neural activation and neuron population in the BLA were investigated 2 h later.RESULTS:
Administration of apocynin prior to neonatal sevoflurane exposure not only prevented learning deficits but also preserved c-Fos-expressing glutamatergic neurons in the BLA.CONCLUSIONS:
Apocynin mitigates the cognitive impairment induced by neonatal sevoflurane exposure and preserves c-Fos-expressing glutamatergic neurons in the basolateral amygdala.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Pediatria
/
Roedores
/
Encéfalo
/
Apoptose
/
Transtornos Cognitivos
/
Superóxidos
/
Memória de Longo Prazo
/
Complexo Nuclear Basolateral da Amígdala
/
Anestesia
/
Anestésicos
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Korean Journal of Anesthesiology
Ano de publicação:
2017
Tipo de documento:
Artigo
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