Splenectomy affects the balance between hepatic growth factor and transforming growth factor-beta and its effect on liver regeneration is dependent on the amount of liver resection in rats / 대한외과학회지
Journal of the Korean Surgical Society
;
: 238-245, 2012.
Artigo
em Inglês
| WPRIM
| ID: wpr-15807
ABSTRACT
PURPOSE:
Small-for-size syndrome (SFSS) is a major problem in liver surgery, and splenectomy has been used to prevent SFSS. However, it is unknown whether splenectomy has the same effect on liver regeneration in both standard and marginal hepatectomy. The aim of this study is to see a difference in effect of splenectomy on liver regeneration according to the amount of liver resection.METHODS:
Thirty male Sprague-Dawley rats (220 to 260 g) were divided into the following five groups control (n = 6), 70% hepatectomy (n = 6), 70% hepatectomy with splenectomy (n = 6), 90% hepatectomy (n = 6), and 90% hepatectomy with splenectomy (n = 6). The animals were euthanized 24 hours after surgery and liver specimens were obtained. To assess liver regeneration, we performed immunohistochemistry of liver tissue using 5-bromo-2-deoxyuridine (BrdU) labeling and Western blot analysis of hepatic growth factor (HGF) and transforming growth factor-beta (TGF-beta) in the liver tissue.RESULTS:
The splenectomized subgroup had a higher BrdU-positive cell count in the 90% hepatectomy group, but not in the 70% hepatectomy group (P < 0.001). Splenectomy significantly decreased TGF-beta expression (P = 0.005) and increased the HGF to TGF-beta ratio (P = 0.002) in the 90% hepatectomy group, but not in the 70% hepatectomy group.CONCLUSION:
The positive effect of splenectomy on liver regeneration was greater in the group with the larger liver resection. This phenomenon may be related to the relative balance between HGF and TGF-beta in the liver.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Esplenectomia
/
Imuno-Histoquímica
/
Contagem de Células
/
Western Blotting
/
Fator de Crescimento Transformador beta
/
Ratos Sprague-Dawley
/
Hepatectomia
/
Fígado
/
Regeneração Hepática
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Journal of the Korean Surgical Society
Ano de publicação:
2012
Tipo de documento:
Artigo
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