Development of Artificial Vessels with Autologous Bone Marrow Cells and Polymers / 대한흉부외과학회지
The Korean Journal of Thoracic and Cardiovascular Surgery
;
: 160-169, 2008.
Artigo
em Coreano
| WPRIM
| ID: wpr-159172
ABSTRACT
BACKGROUND:
To treat anastomosis site stenosis and occlusion of the artificial vessels used in vascular surgery, tissue-engineered artificial vessels using autologous cells have been constructed. We developed artificial vessels using a polymer scaffold and autologous bone marrow cells and performed an in vivo evaluation. MATERIAL ANDMETHOD:
We manufactured a vascular scaffold using biodegradable PLCL (poly lactide-co-epsilon-caprolactone) and PGA (poly glycolic acid) fibers. Then we seeded autologous bone marrow cells onto the scaffold. After implantation of the artificial vessel into the abdominal aorta, we performed an angiography 3 weeks after surgery. After the dogs were euthanized we retrieved the artificial vessels and performed histological analysis.RESULT:
Among the six dogs, 2 dogs died of massive bleeding due to a crack in the vascular scaffold 10 days after the operation. The remaining four dogs lived for 3 weeks after the operation. In these dogs, the angiography revealed no stenosis or occlusion at 3 weeks after the operation. Gross examination revealed small thrombi on the inner surface of the vessels and the histological analysis showed three layers of vessel structure similar to the native vessel. Immunohistochemical analysis demonstrated regeneration of the endothelial and smooth muscle cell layers.CONCLUSION:
A tissue engineered vascular graft was manufactured using a polymer scaffold and autologous bone marrow cells that had a structure similar to that of the native artery. Further research is needed to determine how to accommodate the aortic pressure.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Aorta Abdominal
/
Polímeros
/
Artérias
/
Prostaglandinas A
/
Regeneração
/
Sementes
/
Medula Óssea
/
Prótese Vascular
/
Células da Medula Óssea
/
Angiografia
Limite:
Animais
Idioma:
Coreano
Revista:
The Korean Journal of Thoracic and Cardiovascular Surgery
Ano de publicação:
2008
Tipo de documento:
Artigo
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