p53 Mutation of Head and Neck Squamous Cell Carcinoma Cell Lines / 대한암학회지
Journal of the Korean Cancer Association
;
: 12-19, 1998.
Artigo
em Coreano
| WPRIM
| ID: wpr-15946
ABSTRACT
PURPOSE:
Structural alterations of p53 and overexpression of the p53 protein are the most common genetic abnormalities in various kinds of human cancers. In this study, we examined the mutational status and the frequency of p53 mutations in head and neck squamous cell carcimona (HNSCC) cell lines. MATERIALS ANDMETHODS:
7 human head and neck squamous cell carcinoma cell lines were included in this analysis. Using polymerase chain reaction(PCR), single strand confonmation polymorphism(SSCP) and PCR-DNA sequencing analysis, we tested the mutational status of 7 cell lines. Exon 4~9 of the p53 gene was amplified for the direct DNA sequencing analysis.RESULTS:
Our results showed 100% nuclear p53 immunostaining and 3 electrophoretic abnomalities by PCR-SSCP in three cancer cell lines and mutations of the p53 gene including 2 base substitutions and 1 base deletion were detected in 3 cancer cell lines using PCR directed DNA sequencing analysis.CONCLUSION:
7 HNSCC cell lines examined in this study provide excellent systems for study of gene therapy using p53 gene.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Carcinoma de Células Escamosas
/
Terapia Genética
/
Linhagem Celular
/
Reação em Cadeia da Polimerase
/
Éxons
/
Genes p53
/
Análise de Sequência de DNA
/
Cabeça
/
Pescoço
Limite:
Humanos
Idioma:
Coreano
Revista:
Journal of the Korean Cancer Association
Ano de publicação:
1998
Tipo de documento:
Artigo
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