The Study of X Chromosome Inactivation Mechanism in Klinefelter's Syndrome by cDNA Microarray Experiment
Genomics & Informatics
;
: 30-35, 2004.
Artigo
em Inglês
| WPRIM
| ID: wpr-160478
ABSTRACT
To investigate the XIST gene expression and its effect in a Klinefelter''s patient, we used Klinefelter''s syndrome (XXY) patient with azoospermia and also used a normal male (XY) and a normal female (XX) as the control, We were performed cytogenetic analysis, Y chromosomal microdeletion assay (Yq), semi-quantitative RT-PCR, and the Northern blot for Klinefelter''s syndrome (KS) patient, a female and a male control, We extracted total RNA from the KS patient, and from the normal cells of the female and male control subjects using the RNA prep kit (Qiagen), cDNA microarray contained 218 human X chromosome-specific genes was fabricated. Each total RNA was reverse transcribed to the first strand cDNA and was labeled with Cy-3 and Cy-5 fluorescein, The microarray was scanned by ScanArray 4000XL system. XIST transcripts were detected from the Klinefelters patient and the female by RT-PCR and Northern blot analysis, but not from the normal male, In the cDNA microarray experiment, we found 24 genes and 14 genes are highly expressed in KS more than the normal male and females, respectively. We concluded that highly expressed genes in KS may be a resulted of the abnormal X inactivation mechanism.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Cromossomo X
/
RNA
/
Expressão Gênica
/
Northern Blotting
/
DNA Complementar
/
Fluoresceína
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Análise Citogenética
/
Inativação do Cromossomo X
/
Azoospermia
Limite:
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Genomics & Informatics
Ano de publicação:
2004
Tipo de documento:
Artigo
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