Reduced Autophagy in 5-Fluorouracil Resistant Colon Cancer Cells
Biomolecules & Therapeutics
;
: 315-320, 2017.
Artigo
em Inglês
| WPRIM
| ID: wpr-160698
ABSTRACT
We investigated the role of autophagy in SNUC5/5-FUR, 5-fluorouracil (5-FU) resistant SNUC5 colon cancer cells. SNUC5/5-FUR cells exhibited low level of autophagy, as determined by light microscopy, confocal microscopy, and flow cytometry following acridine orange staining, and the decreased level of GFP-LC3 puncta. In addition, expression of critical autophagic proteins such as Atg5, Beclin-1 and LC3-II and autophagic flux was diminished in SNUC5/5-FUR cells. Whereas production of reactive oxygen species (ROS) was significantly elevated in SNUC5/5-FUR cells, treatment with the ROS inhibitor N-acetyl cysteine further reduced the level of autophagy. Taken together, these results indicate that decreased autophagy is linked to 5-FU resistance in SNUC5 colon cancer cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Autofagia
/
Laranja de Acridina
/
Espécies Reativas de Oxigênio
/
Colo
/
Neoplasias do Colo
/
Microscopia Confocal
/
Cisteína
/
Citometria de Fluxo
/
Fluoruracila
/
Microscopia
Idioma:
Inglês
Revista:
Biomolecules & Therapeutics
Ano de publicação:
2017
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS