The Regulation of MMP-2 and -14 Expressions by TGF-beta in Lens Epithelial Cells
Journal of the Korean Ophthalmological Society
;
: 1110-1116, 2006.
Artigo
em Coreano
| WPRIM
| ID: wpr-161313
ABSTRACT
PURPOSE:
TGF-beta is a key regulator of epithelial-mesenchymal transition. Among the TGF-beta responses, cell migration is closely associated with the expression of matrix metalloproteinases (MMPs). Therefore, we determined which MMPs are regulated by TGF-beta and examined the TGF-beta signaling involved in this event, focusing on Src family tyrosine kinases (SFKs)METHODS:
First we examined the expression of MMPs in rat lens explant culture treated with TGF-beta and LECs attached to the anterior capsules of patients with nuclear (N), anterior polar (AP) cataracts using RT-PCR and immunofluorescence staining. It was examined whether the expression of MMPs is regulated by SFKs.RESULTS:
The study using RT-PCR and immunofluorescence staining showed the expression of MMP-2 and -14 in explants and the expression of MMP-14 LECs of AP cataracts. The expression of MMP-2 and -14 was blocked by PP2 in explants. Furthermore, the activated form of SFKs was observed in LECs of AP cataracts by immunofluorescence staining.CONCLUSIONS:
We suggest a novel role of SFKs signaling in the expression of MMP-14 induced by TGF-beta.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Catarata
/
Cápsulas
/
Movimento Celular
/
Fator de Crescimento Transformador beta
/
Imunofluorescência
/
Quinases da Família src
/
Metaloproteinases da Matriz
/
Células Epiteliais
/
Transição Epitelial-Mesenquimal
Limite:
Animais
/
Humanos
Idioma:
Coreano
Revista:
Journal of the Korean Ophthalmological Society
Ano de publicação:
2006
Tipo de documento:
Artigo
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