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Influence of Activin and Follistatin on Expression of Insulin-like Growth Factor (IGF)-I, II, Insulin-like Growth Factor Binding Protein (IGFBP)-1, 2, and 3 mRNA in Cultured Mouse Granulosa Cells / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology ; : 592-599, 2003.
Artigo em Coreano | WPRIM | ID: wpr-161659
ABSTRACT

OBJECTIVE:

To investigate the influence of activin and follistatin on the expression of IGF (insulin-like growth factor)-I, II, IGFBP (insulin-like growth factor binding protein)-1, 2, and 3 mRNA in cultured mouse granulosa cells MATERIALS AND

METHODS:

The granulosa cells were obtained from the mouse and cultured for 6 days with 10 ng/ml of activin, 10 ng/ml of follistatin, and 10 ng/ml of activin with 10 ng/m of follistatin, respectively. The cells not treated with activin or follistatin served as control. Reverse transcription-polymerase chain reaction (RT-PCR) has been used to examine the expression of IGF-I, II, IGFBP-1, 2, and 3 mRNA. Results were analyzed with Kolmogorov-Smirnov test and analysis of variance (ANOVA) and statistical significance was defined as p<0.05.

RESULTS:

The expression of IGF-I and II mRNA were not different significantly. However, the expression of IGFBP-3 mRNA was significantly increased in the follistatin group compared to the control group (p<0.05) and significantly decreased in the activin with follistatin group compared to the control group (p<0.05). The expression of IGFBP-1 mRNA was seemed to be increased in the activin group and decreased in the follistatin group compared to the control group, respectively (p=0.07, p=0.07). The expression of IGFBP-2 and 3 mRNA were seemed to be decreased in the activin group compared to the control group (p=0.06, p=0.07, respectively).

CONCLUSION:

Activin and follistatin might play a role as regulators of mouse ovarian physiology by modulating the IGF system, especially IGFBPs.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fisiologia / RNA Mensageiro / Fator de Crescimento Insulin-Like I / Proteínas de Transporte / Proteínas de Ligação a Fator de Crescimento Semelhante a Insulina / Proteína 1 de Ligação a Fator de Crescimento Semelhante à Insulina / Proteína 2 de Ligação a Fator de Crescimento Semelhante à Insulina / Proteína 3 de Ligação a Fator de Crescimento Semelhante à Insulina / Ativinas / Folistatina Limite: Animais Idioma: Coreano Revista: Korean Journal of Obstetrics and Gynecology Ano de publicação: 2003 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fisiologia / RNA Mensageiro / Fator de Crescimento Insulin-Like I / Proteínas de Transporte / Proteínas de Ligação a Fator de Crescimento Semelhante a Insulina / Proteína 1 de Ligação a Fator de Crescimento Semelhante à Insulina / Proteína 2 de Ligação a Fator de Crescimento Semelhante à Insulina / Proteína 3 de Ligação a Fator de Crescimento Semelhante à Insulina / Ativinas / Folistatina Limite: Animais Idioma: Coreano Revista: Korean Journal of Obstetrics and Gynecology Ano de publicação: 2003 Tipo de documento: Artigo