Effects of Corneal Neovascularization on TNF-alpha and TGF-beta 2 mRNA Expression in Endotoxin Induced Uveitis
Journal of the Korean Ophthalmological Society
;
: 1503-1509, 2002.
Artigo
em Coreano
| WPRIM
| ID: wpr-162004
ABSTRACT
PURPOSE:
We tried to evaluate the expression of TNF-alpha and TGF-beta 2 mRNA during the course of the endotoxin induced uveitis (EIU) in rat after inducing neovascularization on cornea.METHODS:
Reverse transcription followed by polymerase chain reaction amplification was used to determine relative mRNA level in two ocular tissues (iris-ciliary body, cornea) at 0, 1, 3, 6, 12, 48 hours after subcutaneous injection of 200 ug of Escherichia coli endotoxin.RESULT:
Rat with corneal neovascularization showed somewhat different pattern of expression. TGF-beta 2 mRNA expression was showed some fluctuations in cornea. TGF-beta 2 mRNA expression in cornea was decreased within 3 hours after endotoxin treatment and increased gradually after 6 hours until 48 hours.CONCLUSION:
TGF-beta2 might be one of the important cytokines in EIU, especially animals with neovascularization on cornea.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Uveíte
/
RNA Mensageiro
/
Reação em Cadeia da Polimerase
/
Citocinas
/
Fator de Crescimento Transformador beta
/
Fator de Necrose Tumoral alfa
/
Neovascularização da Córnea
/
Córnea
/
Transcrição Reversa
/
Escherichia coli
Limite:
Animais
Idioma:
Coreano
Revista:
Journal of the Korean Ophthalmological Society
Ano de publicação:
2002
Tipo de documento:
Artigo
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