Isolation and Characterization of Chicken NPAS3
Experimental Neurobiology
;
: 71-74, 2010.
Artigo
em Inglês
| WPRIM
| ID: wpr-162263
ABSTRACT
Here we describe characterization of chicken neuronal Per-Arnt-Sim domain 3 (NPAS3) gene during embryogenesis including examinations of expression pattern and function of the gene. RTPCR assay showed that the primary tissue of expression for this gene is the central nervous system (CNS) while RNA in situ hybridization assay confirmed that NPAS3 was expressed in the ventricular zone of developing neural tube as early as Hamburger-Hamilton (HH) stage 20. Ectopic over-expression of the gene in ovo in the developing chicken neural tube by electroporation had little effect on stem cell population, overall neurogenesis, and motor neuron differentiation. We discuss the implications of our observation.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Células-Tronco
/
RNA
/
Sistema Nervoso Central
/
Galinhas
/
Hibridização In Situ
/
Eletroporação
/
Desenvolvimento Embrionário
/
Tubo Neural
/
Neurogênese
/
Neurônios Motores
Limite:
Gravidez
Idioma:
Inglês
Revista:
Experimental Neurobiology
Ano de publicação:
2010
Tipo de documento:
Artigo
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