Genome Size Constraint in Replication and Packaging of Turnip Yellow Mosaic Virus
Journal of Bacteriology and Virology
;
: 188-196, 2014.
Artigo
em Inglês
| WPRIM
| ID: wpr-162998
ABSTRACT
Turnip yellow mosaic virus (TYMV) is a spherical plant virus that has a single 6.3 kb positive strand RNA as a genome. Previously, we have made the recombinant TYMV construct containing a 0.7 kb eGFP gene or a 1.8 kb GUS gene. The genomic RNAs from these constructs were efficiently encapsidated. To examine in more detail whether size constraint exists for replication and packaging of TYMV, we have inserted into the TY-GUS an extra sequence derived from either eGFP or GUS. We also made a recombinant containing RNA1 sequence of Flock house virus. These TYMV recombinants were introduced into Nicotiana benthamiana leaves by agroinfiltration. Northern blot analysis of the viral RNAs in the agroinfiltrated leaves showed that the genomic RNA band from the recombinant TYMV became weaker as longer sequence was inserted. The result also showed that the efficiency of genomic RNA encapsidation decreased sharply when an extra sequence of 2.2 kb or more was inserted. In contrast, the recombinant subgenomic RNA containing an extra sequence of up to 3.2 kb was efficiently encapsidated. Overall, these results show that size constraint exists for replication and encapsidation of TYMV RNA.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Vírus de Plantas
/
Nicotiana
/
RNA
/
RNA Viral
/
Northern Blotting
/
Genoma
/
Tymovirus
/
Embalagem de Produtos
/
Tamanho do Genoma
Idioma:
Inglês
Revista:
Journal of Bacteriology and Virology
Ano de publicação:
2014
Tipo de documento:
Artigo
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