Molecular Genetic Analysis of Cis-AB with Cytosine at nucleotide position 467
Journal of the Korean Society for Microbiology
;
: 13-20, 1999.
Artigo
em Coreano
| WPRIM
| ID: wpr-163015
ABSTRACT
BACKGROUNDS The molecular genetic characteristcs of cis-AB blood group have shown that its allele had C, G, C and C at nucleotide positions (nps) 526, 703, 796 and 803, respectively. And all cis-AB analysed and reported molecular genetically in Korea and Japan had T at np 467 (leucine at amino acid position 156). We report a first case of cis-AB with C at np 467 (proline at amino acid position 156). METHODS:
Genomic DNA was extracted from peripheral blood of cis-AB patient and amplified by DS1/DS2 and DS3/DS4 allele-specific primers. After PCR, we analysed nps 261, 467, 526, 646, 703, 796, and 803 by restriction digestion, autoradiography and automatic sequencing.RESULTS:
PCR-RFLP with DS1/DS2 primers and restriction enzyme KpnI showed that cis-AB had an 0 allele. The results of genomic sequencing, autoradiography and restriction digestion showed that cis-AB allele at nps 467, 526, 646, 703, 796 and 803 had C, C, T, G, C and C, respectively.CONCLUSION:
This cis-AB showed characteristic molecular genetic features at nps 526, 703, 796, and 803. And this is a first case of A(Pro) cis-AB with C at np 467. (Korean J Blood Transfusion 10(1) 13-19, 1999)
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Autorradiografia
/
Transfusão de Sangue
/
DNA
/
Reação em Cadeia da Polimerase
/
Citosina
/
Digestão
/
Alelos
/
Japão
/
Coreia (Geográfico)
/
Biologia Molecular
Limite:
Humanos
País/Região como assunto:
Ásia
Idioma:
Coreano
Revista:
Journal of the Korean Society for Microbiology
Ano de publicação:
1999
Tipo de documento:
Artigo
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