Analysis of Acquired Resistance Genes in Stenotrophomonas maltophilia / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
;
: 295-300, 2010.
Artigo
em Coreano
| WPRIM
| ID: wpr-164234
ABSTRACT
BACKGROUND:
Stenotrophomonas maltophilia is a gram-negative bacillus and a nosocomial pathogen in immunocompromised patients. Trimethoprim/sulfamethoxazole (TMP/SMX) is the drug of choice for treating S. maltophilia infection; however, resistance to TMP/SMX is increasing. In this study, we investigated the relationship between the incidence of TMP/SMX resistance and the presence of sul genes and mobile elements.METHODS:
A total of 120 S. maltophilia isolates were collected from 3 university hospitals between April 2007 and April 2009. Antimicrobial susceptibilities were determined using the disk diffusion method. PCR and DNA sequencing were conducted for the detection of sul1, sul2, class 1 integron, and ISCR2 element. Repetitive extragenic palindromic sequence-based PCR (REP-PCR) was carried out to evaluate the genetic relatedness.RESULTS:
The TMP/SMX-resistant (R) isolates harbored a significantly higher proportion of sul1 gene and class 1 integron than TMP/SMX-susceptible (S) isolates (P<0.001). Seventeen of 28 isolates with sul1 also had a class 1 integron, but none of the isolates without sul1 had a class 1 integron. The identified gene cassettes within class 1 integrons include aacA4, aadA1, aac6'-II, and qac. None of the 120 isolates carried sul2, glmM, or ISCR2 element. REP-PCR did not show any genetic relatedness among the isolates.CONCLUSIONS:
In Korea, the resistance of S. maltophilia isolates to TMP/SMX is due to sul1 within a class 1 integron rather than to sul2. The class 1 integron also harbors multiple antibiotic resistance genes in addition to sul1, and therefore it could mediate multidrug resistance in S. maltophilia.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas de Bactérias
/
DNA Bacteriano
/
Proteínas de Transporte
/
Combinação Trimetoprima e Sulfametoxazol
/
Reação em Cadeia da Polimerase
/
Stenotrophomonas maltophilia
/
Farmacorresistência Bacteriana Múltipla
/
Integrons
/
Testes de Sensibilidade a Antimicrobianos por Disco-Difusão
/
Antibacterianos
Limite:
Humanos
Idioma:
Coreano
Revista:
The Korean Journal of Laboratory Medicine
Ano de publicação:
2010
Tipo de documento:
Artigo
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