Caveolin-1 regulates osteoclast differentiation by suppressing cFms degradation
Experimental & Molecular Medicine
;
: e192-2015.
Artigo
em Inglês
| WPRIM
| ID: wpr-165770
ABSTRACT
Caveolae are flask-shaped cell-surface membranes, which consist of cholesterol, sphingolipids and caveolin proteins. In a microarray analysis, we found that caveolin-1 (Cav-1) was upregulated by receptor activator of NFkappaB ligand (RANKL), the osteoclast differentiation factor. Silencing of Cav-1 inhibited osteoclastogenesis and also decreased the activation of mitogen-activated protein kinase and the induction of NFATc1 by RANKL. Cav-1 knockdown suppressed the expression of cFms and RANK, two major receptors for osteoclastogenesis. Interestingly, cFms expression was decreased only at the protein level, not at the messenger RNA (mRNA) level, whereas RANK expression was decreased at both the mRNA and protein levels. Furthermore, Cav-1 deficiency increased the lysosomal degradation of cFms. Taken together, these results demonstrate that Cav-1-dependent cFms stabilization contributes to efficient osteoclastogenesis.
Texto completo:
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Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Inglês
Revista:
Experimental & Molecular Medicine
Ano de publicação:
2015
Tipo de documento:
Artigo
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