Cholesterol conjugated spermine as a delivery modality of antisense oligonucleotide
International Journal of Oral Biology
;
: 155-160, 2013.
Artigo
em Inglês
| WPRIM
| ID: wpr-166284
ABSTRACT
The major issue in the development of nucleic acid based therapeutics is the inefficient delivery of these agents into cells. We prepared cholesterol conjugated spermine and evaluated its usefulness as a delivery modality for antisense oligonucleotides in HeLa-Luc cells. A 2'-O-methyl antisense oligonucleotide sequence, designed to correct splicing at an aberrant intron inserted into a normal luciferase reporter gene, was used for complex formation with cholesterol conjugated spermine. Effective delivery of this antisense agent into nucleus would results in the expression of a luciferasereporter gene product. The cholesterol-spermine formed stable complexes with the antisense oligonucleotide and showed modest delivery activity. Furthermore, this delivery activity was maintained even in the presence of serum proteins, mimicking in vivo conditions. Cholesterol-spermine thus has potential as a delivery system for antisense oligonucleotides into cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Sanguíneas
/
Íntrons
/
Espermina
/
Colesterol
/
Oligonucleotídeos Antissenso
/
Genes Reporter
/
Luciferases
Idioma:
Inglês
Revista:
International Journal of Oral Biology
Ano de publicação:
2013
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS