Antifilarial and Antibiotic Activities of Methanolic Extracts of Melaleuca cajuputi Flowers
The Korean Journal of Parasitology
;
: 273-280, 2016.
Artigo
em Inglês
| WPRIM
| ID: wpr-166330
ABSTRACT
We evaluated the activity of methanolic extracts of Melaleuca cajuputi flowers against the filarial worm Brugia pahangi and its bacterial endosymbiont Wolbachia. Anti-Wolbachia activity was measured in worms and in Aedes albopictus Aa23 cells by PCR, electron microscopy, and other biological assays. In particular, microfilarial release, worm motility, and viability were determined. M. cajuputi flower extracts were found to significantly reduce Wolbachia endosymbionts in Aa23 cells, Wolbachia surface protein, and microfilarial release, as well as the viability and motility of adult worms. Anti-Wolbachia activity was further confirmed by observation of degraded and phagocytized Wolbachia in worms treated with the flower extracts. The data provided in vitro and in vivo evidence that M. cajuputi flower extracts inhibit Wolbachia, an activity that may be exploited as an alternative strategy to treat human lymphatic filariasis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Bioensaio
/
Técnicas In Vitro
/
Filariose Linfática
/
Microscopia Eletrônica
/
Reação em Cadeia da Polimerase
/
Brugia pahangi
/
Aedes
/
Wolbachia
/
Melaleuca
/
Flores
Limite:
Adulto
/
Humanos
Idioma:
Inglês
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2016
Tipo de documento:
Artigo
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