Genetic and Epigenetic Alterations of the Wnt/beta-catenin Signaling Pathway in Cancer of the Ampulla of Vater
Korean Journal of Pathology
; : 224-231, 2007.
Article
em En
| WPRIM
| ID: wpr-16693
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND: Carcinoma of the ampulla of Vater is rare and its pathogenesis is unclear. The role of epigenetic changes in the APC or CDH1, in the Wnt pathway, has not been reported in ampullary carcinomas. METHODS: We performed immunohistochemistry on 73 sporadic ampullary carcinomas to identify Wnt-related molecules (APC, beta-catenin, E-cadherin, c-erbB2, cyclin D1) and examined mutations in the CTNNB1, loss of heterozygosity of 5q21, and the methylation status of the CpG island of APC and CDH1. RESULTS: Thirteen tumors (17.8%) showed abnormal nuclear localization of beta-catenin; this was more prominent in the intestinal type than in the pancreaticobiliary type (p=0.01). The loss of APC correlated with the loss of beta-catenin or c-erb B2 (p<0.01). The prognosis was worse in the group with APC loss than when APC was maintained (p<0.05). There was no mutation identified in CTNNB1. Six (24%) out of 25 informative cases had 5q21 allelic loss. CpG island methylation in APC and CDH1 was detected in 33 (45.2%) and 29 (31.5%) cases, respectively. CONCLUSIONS: The absence of mutations in CTNNB1 and the epigenetic alteration of APC and CDH1, might be characteristic changes in the Wnt/beta-catenin signaling pathway during the carcinogenesis of ampullary carcinomas.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Prognóstico
/
Ampola Hepatopancreática
/
Imuno-Histoquímica
/
Caderinas
/
Ciclinas
/
Ilhas de CpG
/
Perda de Heterozigosidade
/
Beta Catenina
/
Epigenômica
/
Via de Sinalização Wnt
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Korean Journal of Pathology
Ano de publicação:
2007
Tipo de documento:
Article