Diagnostic Utility of Polymerase Chain Reaction-Based Clonality Analysis for Immunoglobulin Heavy Chain Gene and T-cell Receptor Gamma Chain Gene Rearrangement in Lymphoid Neoplasms
Korean Journal of Pathology
;
: 461-469, 2001.
Artigo
em Coreano
| WPRIM
| ID: wpr-166949
ABSTRACT
BACKGROUND:
The clonality of lymphoid infiltrates determined by polymerase chain reaction (PCR) for immunoglobulin heavy chain (IgH) or T cell receptor (TCR) genes is not only useful in confirming the diagnosis of malignant lymphoma but also in establishing the lineage of a clonal lymphoid proliferation. We analyzed the efficiency of PCR analyses for IgH and TCRgenes that have been routinely applied for the diagnosis of malignant lymphoma in our laboratory.METHODS:
Paraffin sections of 200 cases were analyzed by seminested PCR. Primers were FRIIIA-LJH/VLJH consensus primer for IgH gene and V-J consensus primer for TCR gene. The cases showing negative results by PCR for TCR gene were further analyzed by multiplex V family primers with heteroduplex analysis.RESULTS:
PCR approach for IgH gene allowed detection of clonality in 100% of cases with false positive rate of 0.3% and false negative rate of 0%. The combination of PCR for TCR consensus primers with multiplex V family primers allowed detection of clonality in 91% of cases with false positive rate of 0.6% and false negative rate of 10.3%.CONCLUSIONS:
Combined analysis of IgH and TCR gene rearragnements by the PCR technique followed by heteroduplex analysis can be a useful diagnostic adjunct to determine the clonality of various lymphoproliferative diseases with high sensitivity. But clinical, morphological and immunophenotypical correlation should be considered to reach the final diagnosis due to a few false positive cases.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Parafina
/
Imunoglobulinas
/
Receptores de Antígenos de Linfócitos T
/
Linfócitos T
/
Rearranjo Gênico
/
Reação em Cadeia da Polimerase
/
Cadeias Pesadas de Imunoglobulinas
/
Genes Codificadores dos Receptores de Linfócitos T
/
Análise Heteroduplex
/
Consenso
Tipo de estudo:
Estudo diagnóstico
/
Guia de Prática Clínica
Limite:
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Pathology
Ano de publicação:
2001
Tipo de documento:
Artigo
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