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Diagnostic Utility of Polymerase Chain Reaction-Based Clonality Analysis for Immunoglobulin Heavy Chain Gene and T-cell Receptor Gamma Chain Gene Rearrangement in Lymphoid Neoplasms
Korean Journal of Pathology ; : 461-469, 2001.
Artigo em Coreano | WPRIM | ID: wpr-166949
ABSTRACT

BACKGROUND:

The clonality of lymphoid infiltrates determined by polymerase chain reaction (PCR) for immunoglobulin heavy chain (IgH) or T cell receptor (TCR) genes is not only useful in confirming the diagnosis of malignant lymphoma but also in establishing the lineage of a clonal lymphoid proliferation. We analyzed the efficiency of PCR analyses for IgH and TCRgenes that have been routinely applied for the diagnosis of malignant lymphoma in our laboratory.

METHODS:

Paraffin sections of 200 cases were analyzed by seminested PCR. Primers were FRIIIA-LJH/VLJH consensus primer for IgH gene and V-J consensus primer for TCR gene. The cases showing negative results by PCR for TCR gene were further analyzed by multiplex V family primers with heteroduplex analysis.

RESULTS:

PCR approach for IgH gene allowed detection of clonality in 100% of cases with false positive rate of 0.3% and false negative rate of 0%. The combination of PCR for TCR consensus primers with multiplex V family primers allowed detection of clonality in 91% of cases with false positive rate of 0.6% and false negative rate of 10.3%.

CONCLUSIONS:

Combined analysis of IgH and TCR gene rearragnements by the PCR technique followed by heteroduplex analysis can be a useful diagnostic adjunct to determine the clonality of various lymphoproliferative diseases with high sensitivity. But clinical, morphological and immunophenotypical correlation should be considered to reach the final diagnosis due to a few false positive cases.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Parafina / Imunoglobulinas / Receptores de Antígenos de Linfócitos T / Linfócitos T / Rearranjo Gênico / Reação em Cadeia da Polimerase / Cadeias Pesadas de Imunoglobulinas / Genes Codificadores dos Receptores de Linfócitos T / Análise Heteroduplex / Consenso Tipo de estudo: Estudo diagnóstico / Guia de Prática Clínica Limite: Humanos Idioma: Coreano Revista: Korean Journal of Pathology Ano de publicação: 2001 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Parafina / Imunoglobulinas / Receptores de Antígenos de Linfócitos T / Linfócitos T / Rearranjo Gênico / Reação em Cadeia da Polimerase / Cadeias Pesadas de Imunoglobulinas / Genes Codificadores dos Receptores de Linfócitos T / Análise Heteroduplex / Consenso Tipo de estudo: Estudo diagnóstico / Guia de Prática Clínica Limite: Humanos Idioma: Coreano Revista: Korean Journal of Pathology Ano de publicação: 2001 Tipo de documento: Artigo