Roles of Dopamine in Proliferation of Gastric-Cancer Cells
Journal of the Korean Gastric Cancer Association
;
: 132-138, 2006.
Artigo
em Coreano
| WPRIM
| ID: wpr-167568
ABSTRACT
PURPOSE:
Dopamine is a neurotransmitter, but in the GIT, the roles of dopamine are a regulator of epithelial cell proliferation, an endogenous protective factor, and a regulator of stomach cancer cell proliferation. By using two different gastric-cancer cell lines, we assessed the effects of dopamine and dopamine receptors on the proliferation of human gastric-cancer cells. MATERIALS ANDMETHODS:
To assess the effects of dopamine and dopamine receptors on the proliferation of human gastric-cancer cells, we investigated cell proliferation and the expression of D1, D2L, and D2S receptor in two gastric-cancer cell lines, SNU 601 and KCU-C2. The effects of dopamine and dopamine receptors on the level of the cell proliferation were determined by staining with an A/H/E (acridine orange, hoechst and ethidium bromide) mixture.RESULTS:
After dopamine treatment, the cell viability was significantly decreased in SNU 601 cells (P<0.05) where the D2L receptor was absent, but not in KCU-C2 cells. After treatment with raclopride, a D2 receptor antagonist, dopamine-dose-dependent inhibition of cell proliferation was observed in SNU 601 cells (P<0.05). After treatment with SCH 23390, a D1 receptor antagonist, dopamine significantly increased cell proliferation in KCU-C2 cells (P<0.05), but inhibited cell proliferation in SNU 601 cells (no D2L receptor).CONCLUSION:
The dopamine signal via the D1 or the D2S receptor inhibited proliferation of gastric-cancer cells, but that via the D2L receptor increased proliferation. These results suggest that the regulatory effects of dopamine in the gastric-cancer cell proliferation may be controlled by using dopamine receptors.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Neoplasias Gástricas
/
Dopamina
/
Linhagem Celular
/
Sobrevivência Celular
/
Receptores Dopaminérgicos
/
Neurotransmissores
/
Racloprida
/
Citrus sinensis
/
Proliferação de Células
/
Células Epiteliais
Limite:
Humanos
Idioma:
Coreano
Revista:
Journal of the Korean Gastric Cancer Association
Ano de publicação:
2006
Tipo de documento:
Artigo
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