Detection of betanodaviruses in apparently healthy aquarium fishes and invertebrates
Journal of Veterinary Science
;
: 369-374, 2006.
Artigo
em Inglês
| WPRIM
| ID: wpr-167599
ABSTRACT
Betanodaviruses are the causative agents of viral nervous necrosis (VNN) in cultured marine fish. A total of 237 apparently healthy aquarium fish, marine (65 species) and freshwater (12 species) fishes and marine invertebrates (4 species), which were stocked in a commercial aquarium in Seoul, South Korea, were collected from November 2005 to February 2006. The brains of the fish and other tissues of the invertebrates were examined by reverse transcriptasepolymerase chain reaction (RT-PCR) and nested PCR to detect betanodavirus. Positive nested PCR results were obtained from the brains of 8 marine fish species (shrimp fish Aeoliscus strigatus, milkfish Chanos chanos, three spot damsel Dascyllus trimaculatus, Japanese anchovy Engraulis japonicus, pinecone fish Monocentris japonica, blue ribbon eel Rhinomuraena quaesita, look down fish Selene vomer, yellow tang Zebrasoma flavesenes), 1 marine invertebrate species (spiny lobster Pamulirus versicolor), and 2 freshwater fish species (South American leaf fish Monocirrhus polyacanthus and red piranha Pygocentrus nattereri). The detection rate in nested PCR was 11/237 (4.64%). These subclinically infected aquarium fish and invertebrates may constitute an inoculum source of betanodaviruses for cultured fishes in the Korean Peninsula.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Infecções por Vírus de RNA
/
RNA Viral
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Crustáceos
/
Nodaviridae
/
Doenças dos Peixes
/
Peixes
/
Coreia (Geográfico)
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2006
Tipo de documento:
Artigo
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