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Molecular Phylogenetics of Trichostrongylus Species (Nematoda: Trichostrongylidae) from Humans of Mazandaran Province, Iran
The Korean Journal of Parasitology ; : 279-285, 2017.
Artigo em Inglês | WPRIM | ID: wpr-168670
ABSTRACT
The present study was performed to analyze molecularly the phylogenetic positions of human-infecting Trichostrongylus species in Mazandaran Province, Iran, which is an endemic area for trichostrongyliasis. DNA from 7 Trichostrongylus infected stool samples were extracted by using in-house (IH) method. PCR amplification of ITS2-rDNA region was performed, and products were sequenced. Phylogenetic analysis of the nucleotide sequence data was performed using MEGA 5.0 software. Six out of 7 isolates had high similarity with Trichostrongylus colubriformis, while the other one showed high homology with Trichostrongylus axei registered in GenBank reference sequences. Intra-specific variations within isolates of T. colubriformis and T. axei amounted to 0–1.8% and 0–0.6%, respectively. Trichostrongylus species obtained in the present study were in a cluster with the relevant reference sequences from previous studies. BLAST analysis indicated that there was 100% homology among all 6 ITS2 sequences of T. colubriformis in the present study and most previously registered sequences of T. colubriformis from human, sheep, and goat isolates from Iran and also human isolates from Laos, Thailand, and France. The ITS2 sequence of T. axei exhibited 99.4% homology with the human isolate of T. axei from Thailand, sheep isolates from New Zealand and Iran, and cattle isolate from USA.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Tailândia / Trichostrongylus / DNA / Cabras / Ovinos / Sequência de Bases / Reação em Cadeia da Polimerase / Bases de Dados de Ácidos Nucleicos / França / Irã (Geográfico) Limite: Animais / Humanos País/Região como assunto: Ásia / Europa / Oceania Idioma: Inglês Revista: The Korean Journal of Parasitology Ano de publicação: 2017 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Tailândia / Trichostrongylus / DNA / Cabras / Ovinos / Sequência de Bases / Reação em Cadeia da Polimerase / Bases de Dados de Ácidos Nucleicos / França / Irã (Geográfico) Limite: Animais / Humanos País/Região como assunto: Ásia / Europa / Oceania Idioma: Inglês Revista: The Korean Journal of Parasitology Ano de publicação: 2017 Tipo de documento: Artigo