miR-9 and let-7g enhance the sensitivity to ionizing radiation by suppression of NFkappaB1
Experimental & Molecular Medicine
;
: 298-304, 2011.
Artigo
em Inglês
| WPRIM
| ID: wpr-168747
ABSTRACT
The activation of nuclear factor-kappa B1 (NFkappaB1) in cancer cells may confer resistance to ionizing radiation (IR). To enhance the therapeutic efficiency of IR in lung cancer, we screened for microRNAs (miRNAs) that suppress NFkappaB1 and observed their effects on radiosensitivity in a human lung cancer cell line. From time series data of miRNA expression in gamma-irradiated H1299 human lung cancer cells, we found that the expression of miR-9 was inversely correlated with that of NFkappaB1. Overexpression of miR-9 down-regulated the level of NFkappaB1 in H1299 cells, and the surviving fraction of gamma-irradiated cells was decreased. Interestingly, let-7g also suppressed the expression of NFkappaB1, although there was no canonical target site for let-7g in the NFkappaB1 3' untranslated region. From these results, we conclude that the expression of miR-9 and let-7g could enhance the efficiency of radiotherapy for lung cancer treatment through the inhibition of NFkappaB1.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Radiação Ionizante
/
Tolerância a Radiação
/
Sequência de Bases
/
Regulação Neoplásica da Expressão Gênica
/
Sobrevivência Celular
/
Alinhamento de Sequência
/
Perfilação da Expressão Gênica
/
MicroRNAs
/
Linhagem Celular Tumoral
/
Subunidade p50 de NF-kappa B
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Experimental & Molecular Medicine
Ano de publicação:
2011
Tipo de documento:
Artigo
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