Double-stranded RNA virus in Korean Isolate IH-2 of Trichomonas vaginalis
The Korean Journal of Parasitology
; : 87-94, 2007.
Article
em En
| WPRIM
| ID: wpr-169040
Biblioteca responsável:
WPRO
ABSTRACT
In this study, we describe Korean isolates of Trichomonas vaginalis infected with double-stranded (ds) RNA virus (TVV). One T. vaginalis isolate infected with TVV IH-2 evidenced weak pathogenicity in the mouse assay coupled with the persistent presence of a dsRNA, thereby indicating a hypovirulence effect of dsRNA in T. vaginalis. Cloning and sequence analysis results revealed that the genomic dsRNA of TVV IH-2 was 4,647 bp in length and evidenced a sequence identity of 80% with the previously-described TVV 1-1 and 1-5, but only a 42% identity with TVV 2-1 and 3 isolates. It harbored 2 overlapping open reading frames of the putative capsid protein and dsRNA-dependent RNA polymerase (RdRp). As previously observed in the TVV isolates 1-1 and 1-5, a conserved ribosomal slippage heptamer (CCUUUUU) and its surrounding sequence context within the consensus 14-nt overlap implied the gene expression of a capsid protein-RdRp fusion protein, occurring as the result of a potential ribosomal frameshift event. The phylogenetic analysis of RdRp showed that the Korean TVV IH-2 isolate formed a compact group with TVV 1-1 and 1-5 isolates, which was divergent from TVV 2-1, 3 and other viral isolates classified as members of the Giardiavirus genus.
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Texto completo:
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Índice:
WPRIM
Assunto principal:
Filogenia
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Tricomoníase
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Trichomonas vaginalis
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Virulência
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RNA Polimerase Dependente de RNA
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RNA de Cadeia Dupla
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RNA Viral
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Dados de Sequência Molecular
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Fases de Leitura Aberta
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Clonagem Molecular
Limite:
Animals
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Female
/
Humans
País/Região como assunto:
Asia
Idioma:
En
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2007
Tipo de documento:
Article