Genetic diversity of porcine reproductive and respiratory syndrome virus in Korea
Journal of Veterinary Science
;
: 115-124, 2013.
Artigo
em Inglês
| WPRIM
| ID: wpr-169637
ABSTRACT
The high genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) has been an obstacle to developing an effective vaccine for porcine reproductive and respiratory syndrome (PRRS). This study was performed to assess the degree of genetic diversity among PRRSVs from Korean pig farms where wasting and respiratory syndrome was observed from 2005 to 2009. Samples from 786 farms were tested for the presence of PRRSV using reverse transcription PCR protocol. A total of 117 farms were positive for type 1 PRRSV while 198 farms were positive for type 2. Nucleotide sequences encoding the open reading frame (ORF) 5 were analyzed and compared to those of various published PRRSV isolates obtained worldwide. Sequence identity of the ORF 5 in the isolates was 81.6~100% for type 1 viruses and 81.4~100% for type 2 viruses. Phylogenetic analysis of the ORF 5 sequences showed that types 1 and 2 PRRSVs from Korea were mainly classified into three and four clusters, respectively. The analyzed isolates were distributed throughout the clusters independent of the isolation year or geographical origin. In conclusion, our results indicated that the genetic diversity of PRRSVs from Korean pig farms is high and has been increasing over time.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Suínos
/
Variação Genética
/
Fases de Leitura Aberta
/
Análise de Sequência de DNA
/
Vírus da Síndrome Respiratória e Reprodutiva Suína
/
Síndrome Respiratória e Reprodutiva Suína
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Análise de Sequência de Proteína
/
República da Coreia
Limite:
Animais
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2013
Tipo de documento:
Artigo
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