Molecular Genetic Analysis of Cis-AB with Cytosine at nucleotide position 467 / 대한수혈학회지
Korean Journal of Blood Transfusion
; : 13-19, 1999.
Article
em Ko
| WPRIM
| ID: wpr-169755
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUNDS: The molecular genetic characteristcs of cis-AB blood group have shown that its allele had C, G, C and C at nucleotide positions (nps) 526, 703, 796 and 803, respectively. And all cis-AB analysed and reported molecular genetically in Korea and Japan had T at np 467 (leucine at amino acid position 156). We report a first case of cis-AB with C at np 467 (proline at amino acid position 156). METHODS: Genomic DNA was extracted from peripheral blood of cis-AB patient and amplified by DS1/DS2 and DS3/DS4 allele-specific primers. After PCR, we analysed nps 261, 467, 526, 646, 703, 796, and 803 by restriction digestion, autoradiography and automatic sequencing. RESULTS: PCR-RFLP with DS1/DS2 primers and restriction enzyme KpnI showed that cis-AB had an O allele. The results of genomic sequencing, autoradiography and restriction digestion showed that cis-AB allele at nps 467, 526, 646, 703, 796 and 803 had C, C, T, G, C and C, respectively. CONCLUSION: This cis-AB showed characteristic molecular genetic features at nps 526, 703, 796, and 803. And this is a first case of A(Pro) cis-AB with C at np 467.
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Texto completo:
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Índice:
WPRIM
Assunto principal:
Autorradiografia
/
DNA
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Reação em Cadeia da Polimerase
/
Citosina
/
Digestão
/
Alelos
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Japão
/
Coreia (Geográfico)
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Biologia Molecular
Limite:
Humans
País/Região como assunto:
Asia
Idioma:
Ko
Revista:
Korean Journal of Blood Transfusion
Ano de publicação:
1999
Tipo de documento:
Article