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T and B Cell Changes with Aging / 대한임상병리학회지
Korean Journal of Clinical Pathology ; : 135-140, 2001.
Artigo em Coreano | WPRIM | ID: wpr-170042
ABSTRACT

BACKGROUND:

Lymphocyte subset analysis in peripheral blood is widely performed to assess the immune status and to diagnose and monitor various diseases. Some lymphocyte subsets are known to change with age, but only a little data about age-related changes for these subsets in healthy individuals have been reported. So we attempted to review changes for these subsets with age.

METHODS:

Lymphocyte subset analysis was performed on 302 subjects, 189 males and 113 females with age group in all decades of life. Two color direct immunofluorescence flow cytometry (FCM) was done using a SimultestTM IMK-Lymphocyte kit (Becton Dickinson, USA), FACScanTM (Becton Dickinson, USA) and FACSCaliburTM (Becton Dickinson, USA). Lymphocyte subsets analysed were T (CD3+) and B cells (CD19+), helper/inducer T (CD4+) and suppressor/cytotoxic T cells (CD8+), and helper/suppressor (CD4+/CD8+) ratio and natural killer (NK) cells (CD3-CD16+/CD56+). The absolute numbers of each subset were calculated from the total lymphocyte counts. The data collected was analysed using SAS 6.12. A P-value of < 0.05 was considered significant.

RESULTS:

There were no statistically significant differences between male and female subjects. The percentage of CD4+ T cells, and the count of NK cells did not show any significant differences among the various age groups. The age-related changes observed in our study were as follows 1) A decrease in the percentage of B cells; 2) A decrease in the counts of lymphocytes, T cells, B cells, CD4+ T cells and CD8+ T cells; 3) An increase in the percentage of NK cells; and 4) An increase in the CD4+/CD8+ ratio.

CONCLUSIONS:

A characteristic of the aging process shows a marked decrease in T and B cells. The age-related increase in the percentage of cells bearing NK markers can be interpreted as a compensatory consequence to cope with the deterioration of the immune system with age.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Envelhecimento / Linfócitos B / Células Matadoras Naturais / Linfócitos / Linfócitos T / Subpopulações de Linfócitos / Contagem de Linfócitos / Técnica Direta de Fluorescência para Anticorpo / Citometria de Fluxo / Sistema Imunitário Limite: Feminino / Humanos / Masculino Idioma: Coreano Revista: Korean Journal of Clinical Pathology Ano de publicação: 2001 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Envelhecimento / Linfócitos B / Células Matadoras Naturais / Linfócitos / Linfócitos T / Subpopulações de Linfócitos / Contagem de Linfócitos / Técnica Direta de Fluorescência para Anticorpo / Citometria de Fluxo / Sistema Imunitário Limite: Feminino / Humanos / Masculino Idioma: Coreano Revista: Korean Journal of Clinical Pathology Ano de publicação: 2001 Tipo de documento: Artigo