Salinomycin inhibited cell proliferation and induced apoptosis in human uterine leiomyoma cells
Obstetrics & Gynecology Science
;
: 501-506, 2014.
Artigo
em Inglês
| WPRIM
| ID: wpr-17030
ABSTRACT
OBJECTIVE:
The aim of this study was to investigate the anti-proliferative effect of the salinomycin in cell proliferation and apoptosis in primary cultured human uterine leiomyoma cells.METHODS:
Cell viability was measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Caspase-3 activity assay and DNA fragmentation assay were performed to determine the effect of apoptosis. The expression of apoptosis regulatory-related proteins was evaluated by western blot.RESULTS:
The cell viability and proliferation of uterine leiomyoma cells were significantly reduced by salinomycin treatment in a dose-dependent manner. DNA fragmentation assay results showed apoptotic cell death after salinomycin incubation. Salinomycin activated caspase-3, -8, and -9, causing apoptosis in uterine leiomyoma cells. Down-regulation of Bcl-2, XIAP, and FLIP with a concomitant increase in Bax, Fas, and DR5 were observed.CONCLUSION:
These results provided the first evidence that salinomycin induce both intrinsic and extrinsic apoptosis. Therefore, salinomycin may be a promising chemopreventive and therapeutic agent against human uterine leiomyoma.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Regulação para Baixo
/
Sobrevivência Celular
/
Western Blotting
/
Morte Celular
/
Apoptose
/
Proliferação de Células
/
Caspase 3
/
Fragmentação do DNA
/
Leiomioma
Limite:
Humanos
Idioma:
Inglês
Revista:
Obstetrics & Gynecology Science
Ano de publicação:
2014
Tipo de documento:
Artigo
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