Cyclooxygenase expressions and response to radiation therapy in uterine cervix cancer / 부인종양
Korean Journal of Gynecologic Oncology
; : 105-111, 2006.
Article
em En
| WPRIM
| ID: wpr-170740
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE: The aim of this study is to clarify the relationship between COX expressions and radioresistance in cervical cancer. METHODS: Patients with cervical cancer treated by primary radiotherapy were selected from the tumor registry of our institution. According to the response to radiotherapy during and after a month of radiation, poor responder and good responder was defined. Immunohistochemical staining was performed by the ABC method using formalin-fixed, paraffin-embedded tissue sections and monoclonal anti-COX-1,2 antibodies. Correlation of COX expression and response to radiation was analyzed. Cell lines derived from human cervical tumors were used: HeLa, HT3, and C33A. Using western blot, COX-1,2 expressions were identified in each cell line. The sensitivity of the cervix cancer cells to radiation was measured using a clonogenic assay. RESULTS: COX-1 and COX-2 expressions were higher in poor responders than good responders. The difference of COX-1 expression between two groups had marginal statistical significance (p=0.099, Fisher's exact test) and COX-2 expression was significantly higher in poor responders (p=0.034, Fisher's exact test). In the clonogenic assay, survival fraction of HeLa and HT-3 cell lines, which have COX-1 and COX-2 activity, was significantly higher than C33A cell line which has no COX activity (p<0.001). CONCLUSION: Our results suggest that the expression of COX in cervical cancer might be deeply associated with the effect of radiation therapy.
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Texto completo:
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Índice:
WPRIM
Assunto principal:
Radioterapia
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Linhagem Celular
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Neoplasias do Colo do Útero
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Colo do Útero
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Western Blotting
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Prostaglandina-Endoperóxido Sintases
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Anticorpos
Limite:
Female
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Humans
Idioma:
En
Revista:
Korean Journal of Gynecologic Oncology
Ano de publicação:
2006
Tipo de documento:
Article