Effect of Anti-Siglec-F Antibody and Reactive Oxygen Species Blocking on Histamine Release in Urinary Bladder of Ovalbumin-Treated Mice / 대한배뇨장애요실금학회지
International Neurourology Journal
;
: 122-125, 2012.
Artigo
em Inglês
| WPRIM
| ID: wpr-170974
ABSTRACT
PURPOSE:
Sialic acid-binding Ig-like lectin (Siglec) is an immune inhibitory receptor that plays a role in the negative regulation of the activation of immune cells. This study aimed to evaluate the effects of anti-Siglec-F on plasma and urinary histamine levels in ovalbumin (OVA)-challenged urinary bladder in mice.METHODS:
Thirty BALB/c mice were used. In group I (control group, n=5), mice were sensitized with OVA and challenged with saline. In group II (OVA challenge group, n=5), OVA was used for intraperitoneal sensitization and intravesical challenge. The challenged mice in group III (control immunoglobulin G [IgG] group, n=5) and those in group IV (anti-Siglec-F group, n=5) were intraperitoneally pretreated with rabbit control IgG or anti-Siglec-F antibody, respectively. In groups V (N-acetylcysteine [NAC] in OVA challenge group, n=5) and VI (control NAC only, n=5), mice were pretreated with NAC.RESULTS:
Urinary histamine concentrations were significantly higher 7 days after intravesical OVA challenge (P<0.01), whereas plasma histamine levels were not. Pretreatment with anti-Siglec-F antibody significantly prevented the increase in urinary histamine release (P<0.05), whereas pretreatment with the IgG antibody control did not. Also, pretreatment of the OVA challenge group with NAC did not affect the histamine concentration in either urine or plasma.CONCLUSIONS:
Systemic anti-Siglec-F treatment showed anti-allergic effects at least on local histamine release, particularly in the lower urinary bladder.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Óvulo
/
Plasma
/
Bexiga Urinária
/
Imunoglobulina G
/
Histamina
/
Ovalbumina
/
Espécies Reativas de Oxigênio
/
Lectinas Semelhantes a Imunoglobulina de Ligação ao Ácido Siálico
/
Liberação de Histamina
Limite:
Animais
Idioma:
Inglês
Revista:
International Neurourology Journal
Ano de publicação:
2012
Tipo de documento:
Artigo
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