Inhibition of clusterin gene expression via shRNA increases chemosensitivity to paclitaxel in xenografted PEOH cells / 대한산부인과학회지
Korean Journal of Obstetrics and Gynecology
;
: 1493-1498, 2007.
Artigo
em Coreano
| WPRIM
| ID: wpr-171690
ABSTRACT
OBJECTIVE:
To evaluate the inhibition of Clusterin gene expression via shRNA decreases proliferation and metastasis and increases chemosensitivity to paclitaxel in xenografted PEOH cells.METHODS:
1 x 10(6) paclitaxel resistant cell lines transduced with Clusterin shRNA in lentiviral inoculated subcutaneously into the flank region of 6 to 8 week-old female nude mice. Parental cells transduced with LacZ was used as a control. Tumor growth was measured twice every week and calculated by using the formula length x width x depth x 0.5236. The mice were sacrificed and examined for Clusterin expression on tumor cells and counted the metastasis sites.RESULTS:
shRNA for Cluaterin works in vivo and it is the in accord with the in vitro results. Although shRNA for Clusterin group showed decreased tumor growth and proliferation it has not statistical significance. But transfection of Clusterin shRNA on PEOH significantly increased paclitaxel-sensitivity (P<0.05).CONCLUSION:
shRNA targeting of the Clusterin gene decreased the ovarian cancer cell's paclitaxel resistance.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Neoplasias Ovarianas
/
Pais
/
Transfecção
/
Expressão Gênica
/
Linhagem Celular
/
Paclitaxel
/
RNA Interferente Pequeno
/
Clusterina
/
Xenoenxertos
/
Camundongos Nus
Limite:
Animais
/
Feminino
/
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Obstetrics and Gynecology
Ano de publicação:
2007
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS