Protective Effect of Catechin on Apoptosis of the Lens Epithelium in Rats with N-methyl-N-nitrosourea-induced Cataracts
Korean Journal of Ophthalmology
; : 101-107, 2010.
Article
em En
| WPRIM
| ID: wpr-171962
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE: To investigate the effect of catechin on apoptotic cell death in the lens epithelium of rats with cataract. METHODS: Cataract was induced by intraperitoneal injection of 100 mg/kg N-methyl-N-nitrosourea (MNU) to ten day-old Sprague-Dawley rats. The neonatal rats were randomly divided into five groups (n=15 in each group): a control group, and four cataract-induction groups, treated with either 0, 50, 100, 200 mg/kg catechin. We performed slit-lamp biomicroscopic analysis, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay, Western-blot for Bcl-2 and Bax, and immunohistochemistry for caspase-3. RESULTS: Apoptotic cell death in lens epithelial cells that increased following cataract formation in rats was suppressed by cathechin. CONCLUSIONS: Catechin inhibited cataract-induced apoptotic cell death in the lens epithelium and may prove useful for the prevention of cataract progression.
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1
Índice:
WPRIM
Assunto principal:
Catarata
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Distribuição Aleatória
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Catequina
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Western Blotting
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Análise de Variância
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Técnicas Imunoenzimáticas
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Ratos Sprague-Dawley
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Apoptose
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Marcação In Situ das Extremidades Cortadas
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Caspase 3
Limite:
Animals
Idioma:
En
Revista:
Korean Journal of Ophthalmology
Ano de publicação:
2010
Tipo de documento:
Article