Over-expression of PTEN Involved in Troglitazone-induced Apoptosis in Human Osteosarcoma Cells / 대한골관절종양학회지
The Journal of the Korean Bone and Joint Tumor Society
;
: 23-29, 2011.
Artigo
em Inglês
| WPRIM
| ID: wpr-172340
ABSTRACT
PURPOSE:
We investigated the effects of phosphatase and tensin homologue deleted on chromosome 10 gene phosphatase and tensin homologue deleted on chromosome 10 gene (PTEN) expression on the cell proliferation and on the responsiveness of troglitazone in osteosarcoma cells. MATERIALS ANDMETHODS:
Western blotting alnalysis was performed to detect the expression of PTEN in U-2OS cells treated with troglitazone. WST (water-soluble tetrazolium) assay was used to evaluate cell proliferation. Flow cytometry was used to determine cell apoptosis. Further, transfection of wild-type PTEN plasmid DNA was used to upregulate PTEN expression.RESULTS:
Troglitazone treatment induced growth inhibition of U2-OS cells in a dose- and time-dependent manner. Troglitazone increased the expression of PTEN in a dose-dependent manner. PTEN upregulation induced by troglitazone treatment resulted in cell growth inhibition and apoptosis in U-2OS cells. PTEN over-expression by plasmid transfection enhanced these effects of troglitazone. Moreover, no changes were observed in the mutant type-PTEN group.CONCLUSION:
Upregulation of PTEN is involved in the inhibition of cell growth and induction of cell apoptosis by troglitazone. Further, PTEN over-expression can cause cell growth inhibition in osteosarcoma cells and these cell growth inhibitions could be enhance by troglitazone treatment.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
Cromossomos Humanos Par 10
/
DNA
/
Transfecção
/
Osteossarcoma
/
Regulação para Cima
/
Western Blotting
/
Cromanos
/
Apoptose
/
Tiazolidinedionas
Limite:
Humanos
Idioma:
Inglês
Revista:
The Journal of the Korean Bone and Joint Tumor Society
Ano de publicação:
2011
Tipo de documento:
Artigo
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