Detection of Micrometastasis in Draining Vein before & after Manipulation and Peritoneal Cavity in Colorectal Cancer by Reverse Transcriptase-Polymerase Chain Reactions for Cytokeratin 20 and Carcinoembryonic Antigen
Journal of the Korean Surgical Society
;
: 194-198, 2004.
Artigo
em Coreano
| WPRIM
| ID: wpr-172432
ABSTRACT
PURPOSE:
Micrometastasis is known as a significant predictor of prognosis in colorectal cancer patients. Recently, reverse transcriptase polymerase chain reaction (RT-PCR) has been applied to detecting micrometastasis. The drainage vein and peritoneum were examined and the micrometastases assessed in a series of colorectal cancer patients.METHODS:
22 patients, who were histologically diagnosed with colorectal cancer, and 8 patients of serosal and peritoneal brushing, were examined using RT-PCR to amplify the mRNAs for two epithelial markers, carcinoembryonic antigen (CEA) and cytokeratin 20 (CK-20).RESULTS:
Among the 22 colorectal cancer patients, the positive rates of CK-20 and CEA mRNAs in the drainage vein were 10 (45%) and 7 (32%), and those of the serosal and peritoneal brushing were 6 (75%) and 5 (63%), respectively.CONCLUSION:
These results suggest that the "no touch isolation technique" might be useful for operations in advanced colorectal cancer patients, and the brushing of the serosal or Douglas pouch can represent the micrometastasis status.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Cavidade Peritoneal
/
Peritônio
/
Prognóstico
/
Veias
/
RNA Mensageiro
/
Neoplasias Colorretais
/
Antígeno Carcinoembrionário
/
Drenagem
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Escavação Retouterina
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Humanos
Idioma:
Coreano
Revista:
Journal of the Korean Surgical Society
Ano de publicação:
2004
Tipo de documento:
Artigo
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