Assessment of Porcine Reproductive and Respiratory Syndrome Virus RNA Load in Sera and Tissues during Acute Infection
Journal of Veterinary Science
;
: 75-85, 2002.
Artigo
em Inglês
| WPRIM
| ID: wpr-172832
ABSTRACT
Porcine reproductive and respiratory syndrome virus (PRRSV) RNA load in sera and tissues during acute phase of infection was evaluated using a PCR- based quantitative assay. More than 80% of infected pigs (21/25) showed the peak level of viral RNA concentrations in serum (up to 8.6 x 108 copies/ml) at day 5 postinfection (PI), and started to clear the virus from the systemic circulation thereafter. Regression analysis using the viral RNA concentrations in sera obtained from days 5 to 14 PI showed that the viral RNA was cleared at the rate of 0.37 log reduction in the number of PRRSV RNA copies per day. It was estimated to be day 27 PI when the viral RNA in the serum of infected pigs becomes undetectable. When correlation analysis was performed between the systemic clearance rate and viral RNA concentrations in tissues of 9 infected pigs obtained at day 14 PI, moderately strong negative correlation was observed in the thymus (r = - 0.62) and brain stem (r = - 0.48), suggesting the capability of host animal to clear PRRSV from the systemic circulation appears to be related to the viral activity in the thymus and brain stem.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Padrões de Referência
/
Suínos
/
Fatores de Tempo
/
Viremia
/
Tronco Encefálico
/
RNA Viral
/
Modelos Logísticos
/
Vírus da Síndrome Respiratória e Reprodutiva Suína
/
Síndrome Respiratória e Reprodutiva Suína
/
Carga Viral
Tipo de estudo:
Estudo prognóstico
/
Fatores de risco
Limite:
Animais
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2002
Tipo de documento:
Artigo
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