Isolation of Subgroup J Avian Leukosis Virus in Korea
Journal of Veterinary Science
; : 71-74, 2002.
Article
em En
| WPRIM
| ID: wpr-172833
Biblioteca responsável:
WPRO
ABSTRACT
Two subgroup J avian leukosis viurses (ALVs) were isolated from broiler breeder flocks, in which myeloid leukosis had occurred. The isolates could be classified as subgroup J ALV. by the positive reaction in polymerase chain reaction (PCR) with primers specific for subgroup J ALV. Two isolates replicated in chicken embryo fibroblast (CEF) cells from the alv6 chicken line in which cells are resistant to subgroup A and E ALVs. In in vitro serum neutralization tests with other subgroup ALVs including ADOL-Hc1, the prototype of subgroup J ALVs isolated in the United States of America, two isolates were partially neutralized by antibody to ADOL-Hc1, indicating that Korean isolates and ADOL-Hc1 may be antigenically related, but not identical. When the PCR was done with a primer pair designed to amplify genes of E element and long terminal repeat of proviral DNA, the PCR product size of one isolate (KOAL-PET) was smaller than that of ADOL-Hc1, suggesting that some sequences in these regions are deleted.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Doenças das Aves Domésticas
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Leucose Aviária
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Testes de Neutralização
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Linhagem Celular
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Galinhas
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Reação em Cadeia da Polimerase
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Vírus da Leucose Aviária
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Coreia (Geográfico)
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Anticorpos Antivirais
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Antígenos Virais
Limite:
Animals
País/Região como assunto:
Asia
Idioma:
En
Revista:
Journal of Veterinary Science
Ano de publicação:
2002
Tipo de documento:
Article