Preventive Effect of Pentoxifylline on Cyclosporine A-Induced Collagen Synthesis in Calf Pulmonary Artery Endothelial Cells
Journal of the Korean Surgical Society
;
: 135-143, 2009.
Artigo
em Inglês
| WPRIM
| ID: wpr-173198
ABSTRACT
PURPOSE:
Cyclosporine A (CsA) is a potent immunosuppressive agent, and it has been used to prevent rejection of transplanted organs and to treat autoimmune diseases. Many side effects of CsA, including various types of endothelial dysfunction, have been reported. Pentoxifylline (PTX) is a non-selective phosphodiesterase inhibitor that is used for the treatment of peripheral vascular diseases.METHODS:
We investigated the effect of CsA on collagen synthesis and clarified whether PTX has protective effects against CsA-induced arterial vasculopathy using calf pulmonary artery endothelial cells. This study was carried out using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, reverse transcription- polymerase chain reaction (RT-PCR), Western blot analysis, nitric oxide (NO) detection, and cyclic guanosine monophosphate (cGMP) enzyme immunoassay.RESULTS:
CsA treatment significantly increased the expression of collagen type I mRNA and protein and decreased the production of NO and cGMP. However, pre-treatment with PTX exerted anticollagen effect by suppressing the CsA-induced formation of collagen, but this effect of PTX was not modulated by NO and cGMP.CONCLUSION:
Based on the present results, it is expected that PTX may have a protective effect against CsA-induced arterial vasculopathy, although the mechanism of PTX needs to be clarified in future studies.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Pentoxifilina
/
Artéria Pulmonar
/
Rejeição em Psicologia
/
Doenças Autoimunes
/
Sais de Tetrazólio
/
Tiazóis
/
RNA Mensageiro
/
Western Blotting
/
Reação em Cadeia da Polimerase
/
Técnicas Imunoenzimáticas
Idioma:
Inglês
Revista:
Journal of the Korean Surgical Society
Ano de publicação:
2009
Tipo de documento:
Artigo
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