Expression of Calcium Transporters According to Dietary Sodium in the Distal Tubule of Rat Kidneys / 대한신장학회지
Korean Journal of Nephrology
;
: 390-397, 2007.
Artigo
em Inglês
| WPRIM
| ID: wpr-173291
ABSTRACT
PURPOSE:
Hypercalciuria is a risk factor of renal calcium stone and proper initial management is dietary salt restriction. But the molecular mechanism responsible for this sodium calcium relationship remains unclear. The present study investigates the relationship between different amount of sodium intake and the expression level of transporters involved in the active calcium transport.METHODS:
Sprague-Dawley rats were randomized into three groups a normal salt group, a low salt group, and a high salt group. Expression of mRNA and protein of transient receptor potential vanilloid (TRPV) 5 and calbindin-D28K was determined by real-time quantitative PCR and western blots, respectively.RESULTS:
Hematocrit and body weight showed no difference among the three groups. High salt diet led to significant increase in the amount of urinary calcium excretion and decreased mRNA expression of calbindin-D28K and TRPV5. Protein abundance of calbindin-D28K and TRPV5 was decreased but the result was statistically insignificant. Low salt diet decreased the amount of urinary calcium excretion without significant difference. Messenger RNA expression and protein abundance of calbindin-D28K and TRPV5 showed no difference, compared to those of normal salt group.CONCLUSION:
These data suggest that high sodium intake increases urinary calcium excretion, which is accompanied by a decreased expression of calbindin-D28K mRNA.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sódio
/
Peso Corporal
/
Proteínas de Ligação ao Cálcio
/
RNA Mensageiro
/
Sódio na Dieta
/
Western Blotting
/
Cálcio
/
Reação em Cadeia da Polimerase
/
Fatores de Risco
/
Ratos Sprague-Dawley
Tipo de estudo:
Estudo de etiologia
/
Fatores de risco
Limite:
Animais
Idioma:
Inglês
Revista:
Korean Journal of Nephrology
Ano de publicação:
2007
Tipo de documento:
Artigo
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