Isolation of neural precursor cells from skeletal muscle tissues and their differentiation into neuron-like cells
Experimental & Molecular Medicine
;
: 483-490, 2007.
Artigo
em Inglês
| WPRIM
| ID: wpr-174055
ABSTRACT
Skeletal muscle contains several precursor cells that generate muscle, bone, cartilage and blood cells. Although there are reports that skeletal muscle-derived cells can trans-differentiate into neural-lineage cells, methods for isolating precursor cells, and procedures for successful neural induction have not been fully established. Here, we show that the preplate cell isolation method, which separates cells based on their adhesion characteristics, permits separation of cells possessing neural precursor characteristics from other cells of skeletal muscle tissues. We term these isolated cells skeletal muscle-derived neural precursor cells (SMNPs). The isolated SMNPs constitutively expressed neural stem cell markers. In addition, we describe effective neural induction materials permitting the neuron-like cell differentiation of SMNPs. Treatment with retinoic acid or forskolin facilitated morphological changes in SMNPs; they differentiated into neuron-like cells that possessed specific neuronal markers. These results suggest that the preplate isolation method, and treatment with retinoic acid or forskolin, may provide vital assistance in the use of SMNPs in cell-based therapy of neuronal disease.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Células-Tronco
/
Tretinoína
/
Colforsina
/
Antígenos de Diferenciação
/
Adesão Celular
/
Diferenciação Celular
/
Separação Celular
/
Células Cultivadas
/
Músculo Esquelético
/
Linhagem da Célula
Limite:
Animais
Idioma:
Inglês
Revista:
Experimental & Molecular Medicine
Ano de publicação:
2007
Tipo de documento:
Artigo
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